FLOW CYTOMETRY AS TOOL IN PLANT SCIENCES, WITH EMPHASIS ON GENOME SIZE AND PLOIDY LEVEL ASSESSMENT

Authors

  • Mickaël Bourge Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198, Gif-sur-Yvette, France
  • Spencer C. Brown Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198, Gif-sur-Yvette, France
  • Sonja Siljak-Yakovlev Ecologie Systématique Evolution, Univ. Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, 91400, Orsay, France

Keywords:

C-value, flow cytometry, genome size, gif nuclear isolation buffer, ploidy level, fluorescence

Abstract

Flow cytometry has become the method of choice to measure the DNA content (genome size) in plants. Ease of sample preparation, fast acquisition, and accurate measurements have made the method popular in the domains of plant cell biology, systematics, evolution, genetics and biotechnology. Although the cell wall is a problem when isolating plant cells, cytometry remains a powerful tool in plant sciences. Based on our 30-years’ experience in this field, this review will focus at first on genome size measurement using simply isolated nuclei: the good practice for acquisition, nuclei isolation, appropriate buffers, kind of tissues to use. The second part will briefly review what kind of measurements it is possible to make in plant cytometry, and for what purpose: base composition, ploidy level, cell cycle, endoreplication, seed screening, and nuclei/chromosomes sorting. We will address troubleshooting. The commonly-used mathematical tools will be discussed.

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Published

25.12.2018

How to Cite

Bourge, M., C. Brown, S., & Siljak-Yakovlev, S. (2018). FLOW CYTOMETRY AS TOOL IN PLANT SCIENCES, WITH EMPHASIS ON GENOME SIZE AND PLOIDY LEVEL ASSESSMENT. Genetics & Applications, 2(2), 1–12. Retrieved from https://genapp.ba/editions/index.php/journal/article/view/98

Issue

Vol. 2 No. 2 (2018)

Section

Reviews

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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