https://genapp.ba/editions/index.php/journal/issue/feed 2023-11-10T09:28:04+00:00 Jasmina Čakar jasmina.hindija@ingeb.unsa.ba Open Journal Systems <p><strong>Genetics &amp; Applications</strong> (G&amp;A) is the official journal of the Institute for Genetic Engineering and Biotechnology (<a title="INGEB" href="http://www.ingeb.unsa.ba" target="_blank" rel="noopener">www.ingeb.unsa.ba</a>), University of Sarajevo (<a href="http://www.unsa.ba/">http://www.unsa.ba/</a>). It is envisaged as an open access international journal issued twice a year in print and electronic (online) format, publishing peer-reviewed articles of novel and significant discoveries in the fields of basic and applied genetics. Special issues or supplements may also be produced from time to time upon agreement with the Editorial Board.</p> <p>Topics covered within Genetics and Applications (G&amp;A) include: </p> <p>Molecular genetics, Cytogenetics, Plant genetics, Animal genetics, Human genetics, Medical genetics, Forensic genetics, Population and evolutionary genetics, Conservation genetics, Genomics and functional genomics, Genetic engineering and biotechnology and Bioinformatics.</p> <p>Authors are encouraged to submit complete, unpublished, original works that are not under review in any other journals. Acceptable papers are those that gather and disseminate fundamental knowledge in all areas of genetics.</p> https://genapp.ba/editions/index.php/journal/article/view/182 2023-09-08T08:41:46+00:00 Ryosuke Ishii ryoten141@gmail.com

<p>Agarose gel electrophoresis is a routinely employed experimental technique for visualizing nucleic acids, typically combined with ethidium bromide or some other molecular dye staining and a UV transilluminator. However, the utilization of a UV transilluminator is restricted due to high cost and safety considerations. In this study, an inexpensive and simple in-house blue Light Emitting Diode (LED) transilluminator was developed using a high-brightness blue LED, carbon resistor, and smartphone charging cable. Its sensitivity for DNA detection was compared with conventional methods. A detection limit of 4 ng/band was confirmed by observing a 100 bp DNA fragment, which is comparable to ethidium bromide staining and UV transilluminator. Additionally, the in-house blue LED transilluminator can be attached to existing electrophoresis chambers, allowing real-time observation of electrophoresis and offering a superior alternative to conventional methods. In conclusion, the developed in-house blue LED transilluminator demonstrates high sensitivity in detecting DNA fragments and holds potential for advancing low-cost research facilities.</p>

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