Intermediate frequency magnetic field at 250.8 kHz does not induce DNA damage or “Adaptive Response” in vitro

Abstract

The aim of this study was to examine whether intermediate frequency (IF)
magnetic field (MF) induces genotoxic effects or play a role in the induction of
adaptive response after combined exposure to IF MF and ionizing radiation in
leukocytes and in adrenocortical carcinoma cell line H295R. Cells were exposed
to 250.8 kHz at the magnetic field strength of 80 A/m (equivalent to 100 µT
magnetic flux density) for 20 hours, or exposed to IF MF for 20 hours and 24
hours later challenged with ionizing radiation (1.5 Gy X-ray). Evaluation of the
DNA damage was performed with alkaline comet assay. Our results showed that
there was no significant genotoxic effect of IF MF exposure compared to the
controls in both cell types. Furthermore, results did not indicate a statistically
significant change in DNA strand breaks in IF MF pre-exposed cells when they
were subsequently exposed to 1.5 Gy. Consequently no adaptive response was
detected.