Antiaging Effects of Limonene in Ageing-Induced HaCaT Cells

Authors

  • Jülide Secerli Department of Pharmaceutical Toxicology, Gülhane Faculty of Pharmacy, University of Health Sciences Turkey, Ankara, Türkiye
  • Onur Erdem Department of Pharmaceutical Toxicology, Gülhane Faculty of Pharmacy, University of Health Sciences Turkey, Ankara, Türkiye
  • Merve Bacanlı Department of Pharmaceutical Toxicology, Gülhane Faculty of Pharmacy, University of Health Sciences Turkey, Ankara, Türkiye

DOI:

https://doi.org/10.31383/ga.vol7iss1ga04

Keywords:

senescence, hydrogen peroxide, cytotoxicity, genotoxicity, MTT assay

Abstract

Limonene, obtained from citrus oils such as orange, grapefruit, and lemon, is widely used in cosmetics as a scenting agent. It is regarded as safe because it is not mutagenic, carcinogenic, or nephrotoxic to people and has low human toxicity. This study aims to show the beneficial effects of limonene against hydrogen peroxide (H2O2) induced ageing in human keratinocytes (HaCat) cell line. For this purpose, the concentration value at which H2O2 can cause ageing in HaCaT cells was determined as 200 µM by the MTT method. Then, cytotoxic effects of limonene (1, 10, 25, 50, 100, 250 and 500 µg/mL) alone and in aging-induced cells were determined by the MTT method; possible antigenotoxic effects were determined by alkaline single-cell gel electrophoresis (Comet) method. According to the results obtained, limonene, an ingredient in cosmetics, has protective effects against skin aging in HaCaT cells.

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Published

09.05.2023

How to Cite

Secerli, J., Erdem, O., & Bacanlı, M. (2023). Antiaging Effects of Limonene in Ageing-Induced HaCaT Cells. Genetics & Applications, 7(1), 27–34. https://doi.org/10.31383/ga.vol7iss1ga04

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Section

Research Articles