Comparison Of Expression Levels Of Keratin 19 (Krt19) and Transmembrane Serine Protease 4 (Tmprss4) Genes in SU.86.86 and BxPC-3 Pancreatic Cancer Cell Lines

Abstract

In this study, the expression levels of Keratin 19 (KRT 19) and Transmembrane serine protease 4 (TMPRSS4) genes in metastatic pancreatic cancer cell line SU.86.86 and nonmetastatic pancreatic cancer cell line BxPC3 cells were been determined and the capacity of these two genes to be metastatic biomarkers in pancreatic cancer is aimed to be evaluated. Genomic DNA was isolated from BxPC3 and Su8686 cell lines, which we replicated in culture medium, and then cDNA was obtained. By using gene-specific primers, expression levels of KRT 19 and TMPRSS4 genes were determined at the transcriptional level by real time PCR method. At the end of this study TMPRSS4 gene expression was found to be 1.31-fold decreased and KRT19 gene expression was found to be 1234-fold increased in the metastatic pancreatic cancer cell line SU8686. TMPRSS4 gene expression was found to be 2.38-fold decreased and KRT19 gene expression was found to be 183-fold increased in the non-metastatic BXPC3 cell line. In pancreatic cancers, where survival is very low, most of the patients are in the metastatic process at the time of diagnosis. For this reason, there is an urgent need for metastatic biomarkers that can provide information about the prognosis of the disease. In this study, the expression of the KRT19 gene at the transcriptional level was found to be quite high in the metastatic pancreatic cancer cell line. For this reason, it is thought that studies with large patient groups may be beneficial as a metastatic biomarker candidate in pancreatic cancer.